JoVE Logo
Faculty Resource Center

Sign In

Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo

DOI :

10.3791/3921-v

12:32 min

June 26th, 2013

June 26th, 2013

19,424 Views

1Department of Biology, Washington University in St. Louis , 2Division of Biological Science, Graduate School of Science, Nagoya University, 3Department of Psychiatry, Washington University in St. Louis

Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.

Tags

Retrograde Fluorescent Labeling

-- Views

Related Videos

article

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices

article

Single-unit In vivo Recordings from the Optic Chiasm of Rat

article

DiOLISTIC Labeling of Neurons from Rodent and Non-human Primate Brain Slices

article

Surgical Implantation of Chronic Neural Electrodes for Recording Single Unit Activity and Electrocorticographic Signals

article

Simultaneous Recording of Calcium Signals from Identified Neurons and Feeding Behavior of Drosophila melanogaster

article

Recording Electrical Activity from Identified Neurons in the Intact Brain of Transgenic Fish

article

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

article

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes

article

A Guide to In vivo Single-unit Recording from Optogenetically Identified Cortical Inhibitory Interneurons

article

Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved