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Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo (Video) | JoVE

DOI :

10.3791/3921-v

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12:32 min

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June 26th, 2013

June 26th, 2013

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19,415 Views

1Department of Biology, Washington University in St. Louis , 2Division of Biological Science, Graduate School of Science, Nagoya University, 3Department of Psychiatry, Washington University in St. Louis

Retrograde transport of fluorescent dye labels a sub-population of neurons based on anatomical projection. Labeled axons can be visually targeted in vivo, permitting extracellular recording from identified axons. This technique facilitates recording when neurons cannot be labeled through genetic manipulation or are difficult to isolate using 'blind' in vivo approaches.

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Retrograde Fluorescent Labeling

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