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Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination

DOI :

10.3791/51835-v

August 25th, 2014

August 25th, 2014

17,902 Views

1Department of Physiology and Neurobiology, University of Connecticut, 2Stem Cell Institute, University of Connecticut, 3Department of Neurology, Yale University School of Medicine

A technique to study NG2 cells and oligodendrocytes using a slice culture system of the forebrain and cerebellum is described. This method allows examination of the dynamics of proliferation and differentiation of cells within the oligodendrocyte lineage where the extracellular environment can be easily manipulated while maintaining tissue cytoarchitecture.

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Organotypic Slice Cultures

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