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Organotypic Hippocampal Slice Cultures

DOI :

10.3791/2462-v

February 3rd, 2011

February 3rd, 2011

36,053 Views

1Department of Physiology and Biophysics, University of Washington School of Medicine

We describe a method to prepare organotypic hippocampal slices that can be easily adapted to other brain regions. Brain slices are laid on porous membranes and culture media is allowed to form an interface. This method preserves the gross architecture of the hippocampus for up to 2 weeks in culture.

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Organotypic Hippocampal Slice Cultures

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