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Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals

8.7K Views

12:01 min

October 1st, 2014

DOI :

10.3791/51925-v

October 1st, 2014


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Keywords Lamprey Reticulospinal Axons

Chapters in this video

0:05

Title

1:56

Acute Dissociation of Lamprey Spinal Cord to Yield Isolated Reticulospinal Axons

5:26

Labeling and Identification of Presynaptic Terminals with FM 1-43

6:15

Electrophysiological Recording

7:51

Immunohistochemistry of Isolated Reticulospinal Axons

9:32

Results: Representative Recordings and Immunostaining of an Isolated Reticulospinal Axon

11:21

Conclusion

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