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Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators

DOI :

10.3791/51972-v

November 19th, 2014

November 19th, 2014

14,586 Views

1Department of Physiology, University of California Los Angeles, 2Department of Neurobiology, University of California Los Angeles

The properties and functions of astrocyte intracellular Ca2+ signals in the striatum remain incompletely explored. We describe methods to express genetically encoded calcium indicators in striatal astrocytes using adeno-associated viruses of serotype 2/5 (AAV2/5), as well as procedures to reliably image Ca2+ signals within striatal astrocytes in situ.

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Keywords Intracellular Calcium

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