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Optical Control of a Neuronal Protein Using a Genetically Encoded Unnatural Amino Acid in Neurons

DOI :

10.3791/53818-v

March 28th, 2016

March 28th, 2016

7,805 Views

1Department of Neuroscience, School of Medicine, Tufts University, 2Molecular Neurobiology Laboratory, The Salk Institute for Biological Studies, 3Department of Pharmaceutical Chemistry and the Cardiovascular Research Institute, University of California, San Francisco

Here, a procedure to selectively activate a neuronal protein with a short pulse of light by genetically encoding a photo-reactive unnatural amino acid into a target neuronal protein expressed in neurons in culture or in vivo is presented.

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Optical Control

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