Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators
8.9K Views
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11:33 min
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March 22nd, 2019
DOI :
March 22nd, 2019
•1Japan Science and Technology Agency, PRESTO, 2Laboratory for Developmental Neurobiology, RIKEN Center for Brain Science, 3École Normale Supèrieure, Institut de Biologie de l'ENS (IBENS), Institut national de la santè et de la recherche mèdicale (INSERM), Centre national de la recherche scientifique (CNRS), École Normale Supèrieure, PSL Research University
Chapters in this video
0:04
Title
1:10
Preparation of Poly(ethyleneimine) Coated Coverslips
2:42
Preparation of Rat Cortical Neuron-astrocyte Mixed Culture and Frozen Cells, and the Revival of Frozen Cultures
5:45
Transfection and Adeno-associated Virus Infection of Hippocampal or Cortical Neurons
7:29
Recording Spontaneous Activities of Astrocytes Expressing Lck-RCaMP2 and OER-GCaMP6f
8:37
Results: Dissection of Local Ca2+ Signals in Cultured Cells
10:38
Conclusion
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