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Visualizing and Analyzing Intracellular Transport of Organelles and Other Cargos in Astrocytes

DOI :

10.3791/60230-v

August 28th, 2019

August 28th, 2019

7,531 Views

1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill

Here we describe an in vitro live-imaging method to visualize intracellular transport of organelles and trafficking of plasma membrane proteins in murine astrocytes. This protocol also presents an image analysis methodology to determine cargo transport itineraries and kinetics.

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Keywords Intracellular Transport

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