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JoVE Journal

Developmental Biology

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Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle

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11:22 min

January 12th, 2015

January 12th, 2015

15,743 Views

0:05

Title

1:37

Isolating Muscle-derived Precursor Cells

3:48

Immunomagnetic Bead Sorting of CD56+ Cells

6:17

Image Processing and Analysis of Fluorescently Labeled Nuclear Transcription Factors

8:58

Results: Representative Characterization of Primary Human Skeletal Muscle Myogenic and Fibroblast Cells

10:43

Conclusion

The main adherent cell types derived from human muscle are myogenic cells and fibroblasts. Here, cell populations are enriched using magnetic-activated cell sorting based on the CD56 antigen. Subsequent immunolabelling with specific antibodies and use of image analysis techniques allows quantification of cytoplasmic and nuclear characteristics in individual cells.

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