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Isolation and Characterization of Single Cells from Zebrafish Embryos

DOI :

10.3791/53877-v

March 12th, 2016

March 12th, 2016

14,118 Views

1Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, 2McAllister Heart Institute, University of North Carolina at Chapel Hill, 3Department of Pathology and Laboratory Medicine, University of North Carolina at Chapel Hill

This protocol describes a method for isolating single cells from zebrafish embryos, enriching for cells of interest, capturing zebrafish cells in microfluidic based single cell multiplex systems, and assessing gene expression from single cells.

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Keywords Single Cell Isolation

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