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Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

DOI :

10.3791/55918-v

July 26th, 2017

July 26th, 2017

9,759 Views

1Department of Orthopedic Surgery, Geneva University Hospitals & Faculty of Medicine, 2Department of Basic Neurosciences, Geneva Medical Center, 3Department of Cell Physiology and Metabolism, Geneva Medical Center

Here, we describe a methodology to obtain a pure population of human myoblasts from adult muscle tissue. These cells are used to study in vitro skeletal muscle differentiation and, in particular, to study proteins involved in Ca2+ signaling.

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Isolation Of Human Myoblasts

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