JoVE Logo
Faculty Resource Center

Sign In

Application of Fluorescent Nanoparticles to Study Remodeling of the Endo-lysosomal System by Intracellular Bacteria

DOI :

10.3791/52058-v

January 2nd, 2015

January 2nd, 2015

9,806 Views

1Abteilung Mikrobiologie, Fachbereich Biologie/Chemie, Universität Osnabrück

This article describes methods for the synthesis and fluorescent labeling of nanoparticles (NPs). The NPs were applied in pulse-chase experiments to label the endo-lysosomal system of eukaryotic cells. Manipulation of the endo-lysosomal system by activities of the intracellular pathogen Salmonella enterica were followed by live cell imaging and quantified.

Tags

Keywords Fluorescent Nanoparticles

-- Views

Related Videos

article

Multiplex Detection of Bacteria in Complex Clinical and Environmental Samples using Oligonucleotide-coupled Fluorescent Microspheres

article

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

article

Single Cell Measurements of Vacuolar Rupture Caused by Intracellular Pathogens

article

Establishment of an In vitro System to Study Intracellular Behavior of Candida glabrata in Human THP-1 Macrophages

article

Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches

article

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling

article

Development of an Economical DNA Delivery System by "Acufection" and its Application to Skin Research

article

System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis

article

Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli

article

A Novel Feeder-free System for Mass Production of Murine Natural Killer Cells In Vitro

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved