Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network Using Functionalized Nanobodies in Cultured Cells

8.9K Views

11:05 min

February 21st, 2019

DOI :

10.3791/59111-v

February 21st, 2019


Transcript

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Nanobody

Chapters in this video

0:04

Title

1:03

Bacterial Liquid Culture and Induction of Functionalized Nanobody Expression

2:31

Purification of Functionalized Nanobodies

4:14

Validation of Functionalized Nanobody Expression

4:41

Uptake of Functionalized Nanobodies by Cultured Cells

8:03

Results: Analysis of Endocytic Uptake and Retrograde Transport to the Trans-Golgi Network

10:09

Conclusion

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