JoVE Logo
Faculty Resource Center

Sign In

Identification of Key Factors Regulating Self-renewal and Differentiation in EML Hematopoietic Precursor Cells by RNA-sequencing Analysis

DOI :

10.3791/52104-v

November 11th, 2014

November 11th, 2014

12,152 Views

1The Vivian L. Smith Department of Neurosurgery, Center for Stem Cell and Regenerative Medicine, University of Texas Health Science Center, The University of Texas Graduate School of Biomedical Sciences at Houston

RNA-sequencing and bioinformatics analyses were used to identify significantly and differentially expressed transcription factors in Lin-CD34+ and Lin-CD34- subpopulations of mouse EMLcells. These transcription factors might play important roles in determining the switch between self-renewing Lin-CD34+ and partially differentiated Lin-CD34- cells.

Tags

RNA sequencing Analysis

-- Views

Related Videos

article

Isolation and Analysis of Hematopoietic Stem Cells from the Placenta

article

The use of SC1 (Pluripotin) to Support mESC Self-renewal in the Absence of LIF

article

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

article

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

article

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

article

Monitoring Functionality and Morphology of Vasculature Recruited by Factors Secreted by Fast-growing Tumor-generating Cells

article

In Vivo 4-Dimensional Tracking of Hematopoietic Stem and Progenitor Cells in Adult Mouse Calvarial Bone Marrow

article

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

article

Fecal Glucocorticoid Analysis: Non-invasive Adrenal Monitoring in Equids

article

Analysis of Cell Suspensions Isolated from Solid Tissues by Spectral Flow Cytometry

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved