Detection of Glycosaminoglycans by Polyacrylamide Gel Electrophoresis and Silver Staining
4.5K Views
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05:57 min
•
February 25th, 2021
DOI :
February 25th, 2021
•Wells B. LaRiviere1,2, Xiaorui Han3,4, Kaori Oshima1, Sarah A. McMurtry1, Robert J. Linhardt3, Eric P. Schmidt1,5
1Department of Medicine, University of Colorado Anschutz Medical Campus, 2Medical Scientist Training Program, University of Colorado Anschutz Medical Campus, 3Department of Chemistry and Chemical Biology, Rensselaer Polytechnic Institute, 4Department of Health Sciences, Curtin University, 5Department of Medicine, Denver Health Medical Center
Chapters in this video
0:04
Introduction
0:55
Polyacrylamide Gel Electrophoresis of Isolated and Purified Glycosaminoglycans
2:48
Silver Staining Protocol
3:55
Results: Sensitivity and Specificity of Method of Detection of GAGs
5:27
Conclusion
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