JoVE Logo
Faculty Resource Center

Sign In

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

DOI :

10.3791/52545-v

12:31 min

February 28th, 2015

February 28th, 2015

14,611 Views

1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.

A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

Tags

Structure switching Aptamers

-- Views

Related Videos

article

Hi-C: A Method to Study the Three-dimensional Architecture of Genomes.

article

Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method

article

Whole-Body Nanoparticle Aerosol Inhalation Exposures

article

Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

article

Non-enzymatic, Serum-free Tissue Culture of Pre-invasive Breast Lesions for Spontaneous Generation of Mammospheres

article

qPCRTag Analysis - A High Throughput, Real Time PCR Assay for Sc2.0 Genotyping

article

Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications

article

The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

article

Tethered Bilayer Lipid Membranes to Monitor Heat Transfer between Gold Nanoparticles and Lipid Membranes

article

Nanoparticle Tracking Analysis for the Quantification and Size Determination of Extracellular Vesicles

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved