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A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

DOI :

10.3791/52545-v

12:31 min

February 28th, 2015

February 28th, 2015

14,637 Views

1711th Human Performance Wing, Human Effectiveness Directorate, Air Force Research Laboratory, Wright-Patterson Air Force Base, 2The Henry M. Jackson Foundation, 3UES, Inc.

A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

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Structure switching Aptamers

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