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Detection of Live Escherichia coli O157:H7 Cells by PMA-qPCR

DOI :

10.3791/50967-v

8:16 min

February 1st, 2014

February 1st, 2014

16,174 Views

1Center for Food Safety and Applied Nutrition, Division of Molecular Biology, Food and Drug Administration

A qPCR assay was developed for detection of Escherichia coli O157:H7 targeting a unique genetic marker, Z3276. The qPCR was combined with propidium monoazide (PMA) treatment for live cell detection. This protocol has been modified and adapted to a 96-well plate format for easy and consistent handling of numerous samples

Tags

Escherichia Coli O157 H7

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