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Preparation of Mica and Silicon Substrates for DNA Origami Analysis and Experimentation

DOI :

10.3791/52972-v

July 23rd, 2015

July 23rd, 2015

13,888 Views

1Department of Chemistry and Biochemistry, University of Notre Dame, 2Department of Chemical and Biomolecular Engineering, University of Notre Dame, 3Department of Chemistry, Physics, and Engineering Studies, Chicago State University, 4Department of Technology, Ivy Tech Community College, South Bend, Indiana

Reproducible cleaning processes for substrates used in DNA origami research are described, including bench-top RCA cleaning and derivatization of silicon oxide. Protocols for surface preparation, DNA origami deposition, drying parameters, and simple experimental set-ups are illustrated.

Tags

Mica Substrate

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