Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
12.4K Views
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11:26 min
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July 31st, 2015
DOI :
July 31st, 2015
•Jack C. Bridge1, Jonathan W. Aylott2, Christopher E. Brightling5, Amir M. Ghaemmaghami3, Alan J. Knox4, Mark P. Lewis6, Felicity R.A.J. Rose1, Gavin E. Morris1
1Division of Drug Delivery and Tissue Engineering, University of Nottingham, 2Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, 3Division of Immunology and Allergy, School of Molecular Medical Sciences, University of Nottingham, 4Division of Respiratory Medicine, School of Clinical Sciences, University of Nottingham, 5NIHR Respiratory Biomedical Research Unit, University of Leicester, 6School of Sport, Exercise, and Health Sciences, Loughborough University
Chapters in this video
0:05
Title
1:49
Electrospinning Biphasic and Aligned Polyethylene Terephthalate Scaffolds
5:34
Sterilization of Scaffolds
6:24
Seeding of Fibroblasts and Epithelial Cells onto Biphasic Scaffolds and Airway Smooth Muscle onto Aligned Scaffolds
8:36
Tri-culture Using a Bioreactor System
9:29
Results: Tri-Culture Cells Continue to Produce Cell Type Specific Proteins
10:20
Conclusion
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