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Employing Digital Droplet PCR to Detect BRAF V600E Mutations in Formalin-fixed Paraffin-embedded Reference Standard Cell Lines

13.2K Views

10:16 min

October 8th, 2015

DOI :

10.3791/53190-v

October 8th, 2015


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Keywords Digital Droplet PCR

Chapters in this video

0:05

Title

1:23

DNA Extraction from FFPE Reference Standard Cell Lines

3:33

Digital Droplet PCR: Droplet Generation

5:56

Digital Droplet PCR: PCR Amplification

7:36

Digital Droplet PCR: Droplet Reading and DNA Mutation Profiling

8:28

Results: ddPCR Detects BRAF V600E Mutations in FFPE Reference Standard Cell Lines

9:20

Conclusion

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