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Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

DOI :

10.3791/53225-v

9:32 min

November 11th, 2015

November 11th, 2015

11,659 Views

1Department of Cardiology, Keio University School of Medicine, 2Department of Emergency and Critical Care Medicine, Keio University School of Medicine

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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Induced Pluripotent Stem Cells

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