Generation of Integration-free Induced Pluripotent Stem Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors

10.5K Views

•

09:45 min

•

January 1st, 2017

DOI :

10.3791/55091-v

January 1st, 2017

•

Wei Wen¹, Jian-Ping Zhang¹, Wanqiu Chen², Cameron Arakaki², Xiaolan Li¹^,², David Baylink², Gary D. Botimer³, Jing Xu¹, Weiping Yuan¹, Tao Cheng¹^,⁴^,⁵^,⁶^,⁷, Xiao-Bing Zhang¹^,²

¹State Key Laboratory of Experimental Hematology, Institute of Hematology and Blood Disease Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, ²Division of Regenerative Medicine, Department of Medicine, Loma Linda University, ³Department of Orthopaedic Surgery, Loma Linda University, ⁴Center for Stem Cell Medicine, Chinese Academy of Medical Sciences, ⁵Department of Stem Cell & Regenerative Medicine, Peking Union Medical College, ⁶Collaborative Innovation Center for Cancer Medicine, ⁷Tianjin Key Laboratory of Blood Cell Therapy and Technology

Summary

Explore More Videos

Keywords Induced Pluripotent Stem Cells

Peripheral Blood Mononuclear Cells

Episomal Vectors

Non integrating Reprogramming

Chapters in this video

0:05

Title

1:01

Isolation of Peripheral Blood Mononuclear Cells (PB MNCs)

3:03

Expansion of PB MNCs in Erythroid Medium

4:07

PB MNCs Nucleofection and Reprogramming

8:00

Results: Generation of Integration-free iPSCs from PB MNCs

8:48

Conclusion

Related Videos

12:06

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

12.4K Views

10:52

Generation of Induced Pluripotent Stem Cells from Frozen Buffy Coats using Non-integrating Episomal Plasmids

12.9K Views

08:36

Generation of Integration-free Human Induced Pluripotent Stem Cells Using Hair-derived Keratinocytes

11.4K Views

09:32

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

11.9K Views

10:03

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

9.7K Views

09:57

Induced Pluripotent Stem Cell Generation from Blood Cells Using Sendai Virus and Centrifugation

14.1K Views

08:36

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

12.4K Views

07:51

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

9.1K Views

12:10

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

10.8K Views

10:48

Integration Free Derivation of Human Induced Pluripotent Stem Cells Using Laminin 521 Matrix

8.5K Views