Genetic Modification of Primary Human Keratinocytes
4:33
Building an Organotypic Cassette
5:09
Organotypic Skin Cultures
7:03
Results: Morphology and Gene Expression Analysis of Genetically Modified Human Epidermis
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Conclusion
The goal of this paper is to provide a comprehensive and detailed protocol on how to generate genetically modified human organotypic skin from epidermal keratinocytes and devitalized human dermis.