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Cultivating a Three-dimensional Reconstructed Human Epidermis at a Large Scale

DOI :

10.3791/61802-v

8:49 min

May 28th, 2021

May 28th, 2021

8,873 Views

1Adolphe Merkle Institute, University of Fribourg, 2Dow Silicones Belgium SRL, 3Department of Biomedical and Specialist Surgical Sciences, University of Ferrara, 4Department of Animal Sciences, Plants for Human Health Institute, North Carolina State University

This protocol describes a straightforward method to cultivate three-dimensional reconstituted human epidermis in a reproducible and robust manner. Additionally, it characterizes the structure-function relationship of the epidermal barrier model. The biological responses of the reconstituted human epidermis upon proinflammatory stimuli are also presented.

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Three dimensional Reconstructed Human Epidermis

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