Stable and Efficient Genetic Modification of Cells in the Adult Mouse V-SVZ for the Analysis of Neural Stem Cell Autonomous and Non-autonomous Effects

9.2K Views

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08:48 min

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February 17th, 2016

DOI :

10.3791/53282-v

February 17th, 2016

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Eva Porlan¹, Beatriz Martí-Prado²^,³, Antonella Consiglio⁴^,⁵, Isabel Fariñas²^,³

¹Cell Division and Cancer Group, Spanish National Cancer Research Centre (CNIO), ²Centro de Investigaciones Biomédicas en Red sobre Enfermedades Neurodegenerativas (CIBERNED), ³Departmento de Biologìa Celular, Universidad de Valencia, ⁴Institut de Biomedicina de la Universitat de Barcelona (IBUB), ⁵Department of Molecular and Translational Medicine, Fibroblast Reprogramming Unit, University of Brescia

Summary

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Cell Cycle independent Infection

Fluorescent Proteins

Cell Autonomous And Non autonomous Effects

Chapters in this video

0:05

Title

0:57

Lentivirus Vector Injection Preparation

2:05

Microinjection of Lentiviral Vector

5:37

Results: Reporter Detection by Immunofluorescence in Sections of Injected Mice

7:18

Conclusion

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