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Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

DOI :

10.3791/53364-v

October 1st, 2015

October 1st, 2015

21,211 Views

1Department of Biology, University of Alabama at Birmingham

Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.

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Bacterial Leaf Infiltration Assay

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