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Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy

DOI :

10.3791/4227-v

October 1st, 2012

October 1st, 2012

11,558 Views

1Department of Immunology, University of Pittsburgh School of Medicine, 2Department of Cell Biology and Physiology, University of Pittsburgh School of Medicine

Methods for purifying the cholesterol binding toxin streptolysin O from recombinant E. coli and visualization of toxin binding to live eukaryotic cells are described. Localized delivery of toxin induces rapid and complex changes in targeted cells revealing novel aspects of toxin biology.

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