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Measuring Bacterial Load and Immune Responses in Mice Infected with Listeria monocytogenes

DOI :

10.3791/3076-v

12:50 min

August 9th, 2011

August 9th, 2011

30,159 Views

1St Vincent’s Institute, Department of Medicine, The University of Melbourne, 2Department of Microbiology and Immunology, The University of Melbourne

Listeria monocytogenes is a model organism for studying immune responses and genetic susceptibility to intracellular bacteria in mice. This method enables one to measure bacterial load and generate single-cell suspensions of the liver and spleen from mice for FACS analysis to determine changes in immune cells due to Listeria infection.

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Bacterial Load

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