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Characterization of Thymus-dependent and Thymus-independent Immunoglobulin Isotype Responses in Mice Using Enzyme-linked Immunosorbent Assay

DOI :

10.3791/57843-v

September 7th, 2018

September 7th, 2018

9,063 Views

1Department of Cell Biology and Neuroscience and Graduate Program in Cellular and Molecular Pharmacology, Rutgers University, 2Department of Cell Biology and Neuroscience, Rutgers University, 3Rutgers Cancer Institute of New Jersey

In this paper, we describe a protocol to characterize T-dependent and T-independent immunoglobulin (Ig) isotype responses in mice using ELISA. This method used alone or in combination with flow cytometry will allow researchers to identify differences in B cell-mediated Ig isotype responses in mice following T-dependent and T-independent antigen immunization.

Tags

Thymus dependent

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