JoVE Logo
Faculty Resource Center

Sign In

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

DOI :

10.3791/53422-v

January 14th, 2016

January 14th, 2016

19,749 Views

1Centro de Biotecnología y Genómica de Plantas (CBGP), Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)-Universidad Politécnica de Madrid

Chromatin immunoprecipitation is a powerful technique for the identification of DNA binding sites of Arabidopsis proteins in vivo. This procedure includes chromatin cross-linking and fragmentation, immunoprecipitation with selective antibodies against the protein of interest, and qPCR analysis of bound DNA. We describe a simple ChIP assay optimized for Arabidopsis plants.

Tags

Chromatin Immunoprecipitation Assay

-- Views

Related Videos

article

Chromatin Immunoprecipitation from Human Embryonic Stem Cells

article

Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells

article

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

article

Chromatin Immunoprecipitation (ChIP) using Drosophila tissue

article

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

article

Efficient Chromatin Immunoprecipitation using Limiting Amounts of Biomass

article

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

article

Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP

article

Genome-wide Mapping of Drug-DNA Interactions in Cells with COSMIC (Crosslinking of Small Molecules to Isolate Chromatin)

article

The ChIP-exo Method: Identifying Protein-DNA Interactions with Near Base Pair Precision

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved