Extraction of Leaf Total Protein and Removal of Free Biotin
4:11
Enrichment of Biotinylated Proteins
5:53
Results: Western Blot of Protein Extracts
6:25
Conclusion
Transcript
NLR immune receptors play a crucial role in mediating plant defense against various pathogens. We describe a TurboID-based proximity labeling method for the identification of interaction partners of Toll/interleukin-1 receptor domain containing NL
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Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.