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Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

DOI :

10.3791/53437-v

December 12th, 2015

December 12th, 2015

8,844 Views

1Graduate School of Frontier Sciences, The University of Tokyo

The goal of this protocol is to demonstrate how to monitor fluorescently-tagged protein dynamics on plant cell surfaces with variable-angle epifluorescence microscopy, showing blinking dots of GFP-tagged PATROL1, a membrane trafficking protein, in the cell cortex of the stomatal complex in Arabidopsis thaliana.

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Real time Imaging

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