JoVE Logo
Faculty Resource Center

Sign In

Light Sheet Fluorescence Microscopy of Plant Roots Growing on the Surface of a Gel

DOI :

10.3791/55044-v

6:41 min

January 18th, 2017

January 18th, 2017

14,220 Views

1Developmental and Cell Biology of Plants, Institute of Science and Technology Austria, 2Bioimaging Facility, Institute of Science and Technology Austria

This protocol shows a plant sample preparation method for light-sheet microscopy. The setup is characterized by mounting the plant vertically on the surface of a gel and letting it grow in controlled bright conditions. This allows long-term observation of plant organ development in standardized conditions.

Tags

Keywords Light Sheet Fluorescence Microscopy

-- Views

Related Videos

article

Time-lapse Imaging of Mitosis After siRNA Transfection

article

Fluorescence-microscopy Screening and Next-generation Sequencing: Useful Tools for the Identification of Genes Involved in Organelle Integrity

article

Electroantennographic Bioassay as a Screening Tool for Host Plant Volatiles

article

Using High Resolution Computed Tomography to Visualize the Three Dimensional Structure and Function of Plant Vasculature

article

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

article

In vivo Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

article

Real-time Imaging of Plant Cell Surface Dynamics with Variable-angle Epifluorescence Microscopy

article

High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

article

Visualizing Stromule Frequency with Fluorescence Microscopy

article

Simultaneous Live Imaging of Multiple Insect Embryos in Sample Chamber-Based Light Sheet Fluorescence Microscopes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved