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Tracking and Quantifying Developmental Processes in C. elegans Using Open-source Tools

DOI :

10.3791/53469-v

December 16th, 2015

December 16th, 2015

8,648 Views

1Buchmann Institute for Molecular Life Sciences and Institute of Biochemistry II, School of Medicine, Goethe University

Here it is shown how to track and quantify developmental processes in C. elegans. The methods presented are based on open-source tools that can be easily implemented. It is demonstrated how to reconstruct 3D cell-shape models, how to manually track subcellular structures, and how to analyze cortical contractile flow.

Tags

Developmental Processes

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