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Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

DOI :

10.3791/53541-v

December 31st, 2015

December 31st, 2015

26,420 Views

1Department of Obstetrics and Gynecology, Section of Gynecologic Oncology, University of Chicago

Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.

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Ovarian Cancer Dissemination

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