High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
29.7K Views
•
11:42 min
•
December 28th, 2015
DOI :
December 28th, 2015
•Chapters in this video
0:05
Title
0:52
Culture Inoculation
3:00
Cell Maintenance: Checking Cell Density and Viability and Passaging Cells
5:11
Transfection
7:50
Expression and Harvest
8:36
Results: High Yield Expression of Appropriately Glycosylated Protein with Transient Transfection
10:20
Conclusion
Related Videos
10:00
Controllable Ion Channel Expression through Inducible Transient Transfection
9.3K Views
09:54
Laboratory Scale Production and Purification of a Therapeutic Antibody
17.3K Views
10:52
Efficient and Scalable Production of Full-length Human Huntingtin Variants in Mammalian Cells using a Transient Expression System
2.4K Views
12:59
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
32.3K Views
07:09
A Convenient and General Expression Platform for the Production of Secreted Proteins from Human Cells
21.1K Views
11:20
Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach
53.6K Views
09:16
A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells
9.8K Views
07:08
Efficient Mammalian Cell Expression and Single-step Purification of Extracellular Glycoproteins for Crystallization
13.7K Views
08:17
Production of High-Yield Adeno Associated Vector Batches Using HEK293 Suspension Cells
2.0K Views
06:41
Transgene Expression in Cultured Cells Using Unpurified Recombinant Adeno-Associated Viral Vectors
2.6K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved