October 16th, 2014
•The expression of recombinant proteins by mammalian systems is becoming an attractive method for producing protein complexes for structural biology. Here we present a simple yet highly efficient expression system using suspension grown mammalian cells to purify protein complexes for structural studies.
Related Videos
A Method For Production of Recombinant mCD1d Protein in Insect Cells.
Staining of Proteins in Gels with Coomassie G-250 without Organic Solvent and Acetic Acid
Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
Optimized Transfection Strategy for Expression and Electrophysiological Recording of Recombinant Voltage-Gated Ion Channels in HEK-293T Cells
A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
A Comparative Analysis of Recombinant Protein Expression in Different Biofactories: Bacteria, Insect Cells and Plant Systems
High Yield Expression of Recombinant Human Proteins with the Transient Transfection of HEK293 Cells in Suspension
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved