JoVE Logo
Faculty Resource Center

Sign In

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

DOI :

10.3791/53732-v

March 3rd, 2016

March 3rd, 2016

14,957 Views

1Center for Childhood Cancer and Blood Diseases, Nationwide Children's Hospital

Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).

Tags

Immunofluorescence Analysis

-- Views

Related Videos

article

A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity

article

ReAsH/FlAsH Labeling and Image Analysis of Tetracysteine Sensor Proteins in Cells

article

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

article

Enrichment of Extracellular Matrix Proteins from Tissues and Digestion into Peptides for Mass Spectrometry Analysis

article

Use of Enzymatic Biosensors to Quantify Endogenous ATP or H2O2 in the Kidney

article

Removal of Exogenous Materials from the Outer Portion of Frozen Cores to Investigate the Ancient Biological Communities Harbored Inside

article

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions

article

Visualizing and Tracking Endogenous mRNAs in Live Drosophila melanogaster Egg Chambers

article

Quantitative Immunoblotting of Cell Lines as a Standard to Validate Immunofluorescence for Quantifying Biomarker Proteins in Routine Tissue Samples

article

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved