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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

DOI :

10.3791/53732-v

March 3rd, 2016

March 3rd, 2016

14,984 Views

1Center for Childhood Cancer and Blood Diseases, Nationwide Children's Hospital

Here we report protocols to detect endogenous and exogenous centromere-kinetochore proteins in human cells and quantify these protein levels at centromeres-kinetochores by indirect immunofluorescent staining through the use of fixation (paraformaldehyde, acetone, or methanol fixation).

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Immunofluorescence Analysis

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