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Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

DOI :

10.3791/52030-v

December 20th, 2014

December 20th, 2014

14,774 Views

1Department of Molecular Genetics, The Ohio State University

Here, a novel quantitative fluorescence assay is developed to measure changes in the level of a protein specifically at centrosomes by normalizing that protein’s fluorescence intensity to that of an appropriate internal standard.

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Quantitative Immunofluorescence Assay

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