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Immunostaining Phospho-epitopes in Ciliated Organs of Whole Mount Zebrafish Embryos

DOI :

10.3791/53747-v

February 19th, 2016

February 19th, 2016

7,838 Views

1Department of Biology, Virginia Commonwealth University, 2Center for Human Disease Modeling, Duke University Medical Center

Techniques are described to immunostain phospho-epitopes in whole zebrafish embryos and then conduct two-color fluorescent confocal localization in cellular structures as small as primary cilia. The techniques for fixing and imaging can define the location and kinetics of the appearance or activation of specific proteins.

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