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Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

DOI :

10.3791/59344-v

9:20 min

May 14th, 2019

May 14th, 2019

12,974 Views

1Department of Population Health and Pathobiology, NC State University College of Veterinary Medicine

This protocol demonstrates sequential immunofluorescence and immunohistochemistry on cryosections from early-stage zebrafish embryos enabling precise colocalization analyses in specific cell populations.

Tags

Sequential Immunofluorescence

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