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siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts

DOI :

10.3791/53995-v

September 20th, 2016

September 20th, 2016

9,735 Views

1Department of Biological Sciences, University of Quebec in Montreal, 2Department of Pharmacology and Therapeutics, McGill University, 3Department of Clinical Sciences, University of Montreal

This protocol describes a method for efficiently transfecting siRNA in freshly isolated human villous cytotrophoblasts using microporation and identifying DNA-protein complexes in these cells. Transfected cells can be monitored by Western blot and EMSA analyses during the 4-day culture time.

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