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Isolation of Primary Mouse Trophoblast Cells and Trophoblast Invasion Assay

DOI :

10.3791/3202-v

January 8th, 2012

January 8th, 2012

22,554 Views

1Obstetrics, Gynecology and Women's Health, University of Missouri

In this protocol, we describe the dissection of placentae from the mouse on pregnancy d10.5, followed by isolation of trophoblast cells using a Percoll gradient. We then demonstrate use of the isolated cells in a matrigel invasion assay.

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