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Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

DOI :

10.3791/54036-v

June 13th, 2016

June 13th, 2016

10,008 Views

1Division of Gastroenterology and Hepatology, Department of Medicine, Johns Hopkins University School of Medicine, 2Department of Surgery, Tohoku University

Bile fluid is a valuable source of extracellular vesicles/exosomes that contain potentially important biomarkers. This protocol represents a robust method to isolate exosomes from human bile for further analyses including miRNA profiling.

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