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Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

DOI :

10.3791/50294-v

June 14th, 2013

June 14th, 2013

31,448 Views

1Department of Pharmacology & Physiology, Drexel University College of Medicine

The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

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