JoVE Logo
Faculty Resource Center

Sign In

Purification and microRNA Profiling of Exosomes Derived from Blood and Culture Media

DOI :

10.3791/50294-v

June 14th, 2013

June 14th, 2013

31,497 Views

1Department of Pharmacology & Physiology, Drexel University College of Medicine

The presence of stable microRNAs (miRNAs) in exosomes has generated immense interest as a novel mode of intercellular communication, for their potential utility as biomarkers and as a route for therapeutic intervention. Here we demonstrate exosome purification from blood and culture media followed by quantitative PCR to identify miRNAs being transported.

-- Views

Related Videos

article

Isolation and Purification of Kinesin from Drosophila Embryos

article

Metabolic Labeling of Newly Transcribed RNA for High Resolution Gene Expression Profiling of RNA Synthesis, Processing and Decay in Cell Culture

article

Manual Isolation of Adipose-derived Stem Cells from Human Lipoaspirates

article

Isolation and Characterization of Exosomes from Skeletal Muscle Fibroblasts

article

Isolation and Culture of Primary Synovial Macrophages and Fibroblasts from Murine Arthritis Tissue

article

Isolation and Identification of Mesenchymal Stem Cells Derived from Adipose Tissue of Sprague Dawley Rats

article

Generation of Patient-Derived Podocytes from Skin Biopsies

article

Author Spotlight: The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue

article

Author Spotlight: Accelerating Research on Bacterial Extracellular Vesicles Separation and Heterogeneity

article

Author Spotlight: Advancements in Cultivating Mouse Hair Cells for Auditory Research

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved