JoVE Logo
Faculty Resource Center

Sign In

Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

DOI :

10.3791/54067-v

11:37 min

June 11th, 2016

June 11th, 2016

16,481 Views

1Microbiology, School of Natural Sciences, National University of Ireland Galway, 2School of Biological Sciences, University of Essex

A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

Tags

Simultaneous DNA RNA Extraction

-- Views

Related Videos

article

Laboratory-determined Phosphorus Flux from Lake Sediments as a Measure of Internal Phosphorus Loading

article

Automated Gel Size Selection to Improve the Quality of Next-generation Sequencing Libraries Prepared from Environmental Water Samples

article

Development of Sulfidogenic Sludge from Marine Sediments and Trichloroethylene Reduction in an Upflow Anaerobic Sludge Blanket Reactor

article

A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters

article

Extraction of Structural Extracellular Polymeric Substances from Aerobic Granular Sludge

article

Extraction of Organochlorine Pesticides from Plastic Pellets and Plastic Type Analysis

article

Extraction and Characterization of Surfactants from Atmospheric Aerosols

article

Preparation of Authigenic Pyrite from Methane-bearing Sediments for In Situ Sulfur Isotope Analysis Using SIMS

article

On-Site Molecular Detection of Soil-Borne Phytopathogens Using a Portable Real-Time PCR System

article

Chemical Isolation, Quantification, and Separation of Skin Lipids from Reptiles

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved