JoVE Logo
Faculty Resource Center

Sign In

Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

DOI :

10.3791/50093-v

April 7th, 2013

April 7th, 2013

19,396 Views

1Department of Biology, New Mexico State University

A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.

Tags

Ribosomal RNA

-- Views

Related Videos

article

Large-Scale Screens of Metagenomic Libraries

article

Protocol for Mosquito Rearing (A. gambiae)

article

In vitro Transcription and Capping of Gaussia Luciferase mRNA Followed by HeLa Cell Transfection

article

Substrate Generation for Endonucleases of CRISPR/Cas Systems

article

RNA-seq Analysis of Transcriptomes in Thrombin-treated and Control Human Pulmonary Microvascular Endothelial Cells

article

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

article

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

article

Physiological Recordings and RNA Sequencing of the Gustatory Appendages of the Yellow-fever Mosquito Aedes aegypti

article

Chitosan/Interfering RNA Nanoparticle Mediated Gene Silencing in Disease Vector Mosquito Larvae

article

TChIP-Seq: Cell-Type-Specific Epigenome Profiling

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved