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Depletion of Ribosomal RNA for Mosquito Gut Metagenomic RNA-seq

DOI :

10.3791/50093-v

April 7th, 2013

April 7th, 2013

19,280 Views

1Department of Biology, New Mexico State University

A ribosomal RNA (rRNA) depletion protocol was developed to enrich messenger RNA (mRNA) for RNA-seq of the mosquito gut metatranscriptome. Sample specific rRNA probes, which were used to remove rRNA via subtraction, were created from the mosquito and its gut microbes. Performance of the protocol can result in the removal of approximately 90-99% of rRNA.

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