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Intravital Imaging of Neutrophil Priming Using IL-1β Promoter-driven DsRed Reporter Mice

DOI :

10.3791/54070-v

June 22nd, 2016

June 22nd, 2016

9,428 Views

1Department of Medical Microbiology and Immunology, University of Toledo College of Medicine and Life Sciences, 2Department of Internal Medicine, Yale University School of Medicine, 3Department of Pathophysiology, Southern Medical University (China)

This current protocol employs fluorescent reporters, in vivo labeling, and intravital imaging techniques to enable monitoring of the dynamic process of neutrophil priming in living animals.

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