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Imaging Mycobacterium tuberculosis in Mice with Reporter Enzyme Fluorescence

DOI :

10.3791/56801-v

10:06 min

February 26th, 2018

February 26th, 2018

7,048 Views

1Department of Microbial Pathogenesis and Immunology, Texas A&M Health Science Center, 2Tuberculosis Research Section, Laboratory of Clinical Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health

We describe the optical imaging of mice infected with Mycobacterium tuberculosis (M. tuberculosis) using reporter enzyme fluorescence (REF). This protocol facilitates the sensitive and specific detection of M. tuberculosis in pre-clinical animal models for pathogenesis, therapeutics and vaccine research.

Tags

Mycobacterium Tuberculosis

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