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Recording Temperature-induced Neuronal Activity through Monitoring Calcium Changes in the Olfactory Bulb of Xenopus laevis

DOI :

10.3791/54108-v

11:08 min

June 3rd, 2016

June 3rd, 2016

6,950 Views

1Institute of Neurophysiology and Cellular Biophysics, Georg-August-Universität Göttingen, 2Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Georg-August-Universität Göttingen, 3DFG Excellence Cluster 171, Georg-August-Universität Göttingen, 4German Hearing Center Hannover

Here we describe a protocol for measuring and analyzing temperature responses in the olfactory bulb of Xenopus laevis. Olfactory receptor neurons and mitral cells are differentially stained, after which calcium changes are recorded, reflecting a sensitivity of some neural networks in the bulb to temperature drops induced at the nose.

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Temperature induced Neuronal Activity

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